Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
نویسندگان
چکیده
منابع مشابه
A Plaque Assay for Enumerating Antigen-sensitive Cells in Delayed-type Hypersensitivity
A general method is described for enumerating antigen-sensitive lymphocytes obtained from individuals having delayed hypersensitivity, in this case from highly tuberculin-sensitive guinea pigs. The method is based on the observation that resting lymphocytes are generally unable to support replication of RNA viruses, whereas antigen-"activated" lymphocytes can. Lymph node lymphocytes from indivi...
متن کاملPreparation of prostate specific antigen standards for immunoradiometric assay
Background: Immunoradiometric assay is one of the most common and precise methods for determination of prostate specific antigen (PSA) in clinical laboratories. Usual use of human serum in routine assays has many disadvantages such as easy contamination and precipitation, instability and unavailability. Thus in order to avoid these problems the artificial matrix was used which acts sim...
متن کاملDelayed-type hypersensitivity to influenza virus. Induction of antigen- specific suppressor T cells for delayed-type hypersensitivity to hemagglutinin during influenza virus infection in mice
Mice infected with A/England/939/69 X A/Puerto Rico/8/34 (Rec 31) influenza virus by aerosol develop significantly lower levels of delayed-type hypersensitivity (DTH) to A/Hong Kong/1/68 X A/Puerto Rico/8/34/ (X31) virus compared to uninfected mice. The suppression of DTH to the hemagglutinin appears to be mediated by suppressor T cells which carry Lyt-1 membrane antigen marker, and not by sy s...
متن کاملRegulation of Delayed Hypersensitivity
Two potential mechanisms for terminating delayed hypersensitivity (DH) reactions have been examined in desensitized guinea pigs. Lack of macrophage responsiveness to lymphokines was sought as an explanation for the reduced ability of these animals to express delayed hypersensitivity. Skin-reactive factor was injected into the skin of desensitized guinea pigs and a control group of similarly imm...
متن کاملGenetic control of delayed type hypersensitivity to Mycobacterium leprae antigen.
The mechanism of genetic control of Mycobacterium leprae-specific delayed type hypersensitivity (DTH) in mice was investigated in terms of footpad swelling response. Studies with various congenic and recombinant inbred strains of mouse revealed that M. leprae-specific DTH was controlled by genes within the I-A subregion. The footpad swelling response showed an antigen-specific pattern, revealin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2013
ISSN: 1940-087X
DOI: 10.3791/4454